Effects of myenteric denervation on extracellular matrix fibers and mast cell distribution in normal stomach and gastric lesions |
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| Authors: | Cássia F Estofolete Carla Botelho-Machado Sebastião R Taboga Sérgio Zucoloto Ana Cláudia Polli-Lopes Cristiane D Gil |
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| Institution: | 1.Department of Anatomy,School of Medicine - FAMERP,S?o José do Rio Preto,Brazil;2.Department of Biology,Institute of Biosciences, Humanities and Exact Sciences, IBILCE-UNESP,S?o José do Rio Preto,Brazil;3.Department of Pathology,School of Medicine, FMRP-USP,Ribeir?o Preto,Brazil |
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| Abstract: | Background In this study the effect of myenteric denervation induced by benzalconium chloride (BAC) on distribution of fibrillar components
of extracellular matrix (ECM) and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine
(MNNG). Rats were divided in four experimental groups: non-denervated (I) and denervated stomach (II) without MNNG treatment;
non-denervated (III) and denervated stomachs (IV) treated with MNNG. For histopathological, histochemical and stereological
analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert's
Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue/Safranin (AB-SAF). |
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