Enhanced CellClassifier: a multi-class classification tool for microscopy images |
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Authors: | Benjamin Misselwitz Gerhard Strittmatter Balamurugan Periaswamy Markus C Schlumberger Samuel Rout Peter Horvath Karol Kozak Wolf-Dietrich Hardt |
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Institution: | 1.Institute of Microbiology,ETH Zurich,Zürich,Switzerland;2.Light Microscopy Centre, Institute of Biochemistry,ETH Zürich,Zürich,Switzerland |
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Abstract: | Background Light microscopy is of central importance in cell biology. The recent introduction of automated high content screening has
expanded this technology towards automation of experiments and performing large scale perturbation assays. Nevertheless, evaluation
of microscopy data continues to be a bottleneck in many projects. Currently, among open source software, CellProfiler and
its extension Analyst are widely used in automated image processing. Even though revolutionizing image analysis in current
biology, some routine and many advanced tasks are either not supported or require programming skills of the researcher. This
represents a significant obstacle in many biology laboratories. |
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