Automated assay for screening the enzymatic release of reducing sugars from micronized biomass |
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Authors: | David Navarro Marie Couturier Gabriela Ghizzi Damasceno da Silva Jean-Guy Berrin Xavier Rouau Marcel Asther Christophe Bignon |
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Affiliation: | 1.UMR 1163 INRA/Universités de Provence et de la Méditerranée,Unité de Biotechnologie des Champignons Filamenteux,Marseille cede 09,France;2.Architecture et Fonction des Macromolécules Biologiques, UMR 6098,CNRS et université d'Aix-Marseille I et II,Marseille cedex 09,France;3.UMR 1208 INRA/SupAgro/CIRAD/Université Montpellier 2,Unité Ingéniérie des Agropolymères et Technologies Emergentes,Montpellier cedex 01,France |
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Abstract: | Background To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-throughput screening methods. Several methods have been devised all using microplates in the industrial SBS format. Although this size reduction and standardization has greatly improved the screening process, the published methods comprise one or more manual steps that seriously decrease throughput. Therefore, we worked to devise a screening method devoid of any manual steps. |
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