A linearization method for low catalytic activity enzyme kinetic analysis

A kinetic analysis was made and a linear plot based on the general rate equation derived by Laidler Can. J. Chem. 33, 1614-1624] is proposed. This linearization method allows determining the kinetic parameters (K(m), k(cat)) and E](0) for enzymes with low catalytic activity. The method was applied to chloroperoxidase from Caldariomyces fumago EC 1.11.1.10], whose kinetic parameters K(m)(app), k(cat)(app), and E](0) with monochlorodimedone as substrate, were obtained by using the linearization plot and the V(max) value (calculated by Eadie-Hofstee plot). This plot could also be useful to the study of abenzyme kinetics provided the concentration of the latter is either higher or equal than K(m) value.