HLA-DRB1*0410-restricted recognition of XAGE-1b37-48 peptide by CD4 T cells |
| |
Authors: | Morishita Yoshiyuki Uenaka Akiko Kaya Savas Sato Shuichiro Aji Toshiki Nakayama Eiichi |
| |
Institution: | Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Okayama 700-8558, Japan. ymori@jichi.ac.jp |
| |
Abstract: | XAGE-1b belongs to cancer/testis (CT) antigens, and has been shown to be expressed frequently in lung cancers and to elicit an antibody response in patients with XAGE-1b-expressing tumors. In this study, we investigated an XAGE-1b peptide recognized by CD4 T cells. CD4 T cells were purified from PBMC of a healthy donor and stimulated with pooled 25-mer peptides overlapped with 15 amino acids spanning the entire XAGE-1b protein. The generation of XAGE-1b-specific CD4 T cells was shown by IFNgamma secretion assay. A CD4 T cell clone OHD1 was obtained by limiting dilution. OHD1 recognized two overlapping peptides, XAGE1-b(33-49) and XAGE-1b(37-52), by ELISPOT assay. A peptide XAGE-1b(38-46) which was included in both XAGE-1b(33-49) and XAGE-1b(37-52) was predicted to be a DRB1*0410-restricted 9-mer peptide by a computer-based program. We identified the 12-mer peptide XAGE-1b(37-48) as a new XAGE-1b epitope restricted to HLA-DRB1*0410. |
| |
Keywords: | cancer/testis antigen XAGE‐1b CD4 T cell epitope HLA‐DRB1*0410 restriction |
本文献已被 PubMed 等数据库收录! |
|