Abstract: | Optimal cyclic photophosphorylation with reduced indophenols under anaerobic conditions was shown to require a critical redox balance. Over-reduction inhibited this phosphorylation; addition of oxidizing agents like ferricyanide, air, ferredoxin or ferredoxin plus triphosphopyridine nucleotide relieved the inhibition. When ascorbate and indophenol served as the electron donor couple for TPN+ reduction, only the amount of TPNH formed was dependent on the concentration of TPN+. The phosphorylation observed in this system was dependent only on the concentration of indophenol, and on the ability of reduced indophenol to mediate cyclic photophosphorylation. The cyclic electron flow with reduced indophenol was shown to operate simultaneously with the non-cyclic electron flow to TPN+. It was concluded that there was no phosphorylation site in the non-cyclic electron flow between ascorbate-indophenol and TPN+ and that the phosphorylation observed in this case was due only to cyclic photophosphorylation with the reduced indophenols. In the light of these results, a working hypothesis with two different sites for cyclic and non-cyclic photophosphorylation is suggested. |