Mutational specificity of thymine deprivation-induced mutation in the lacI gene of Escherichia coli |
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Authors: | B W Glickman F L Allen and M J Horsfall |
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Institution: | Department of Biology, York University, 4700 Keele Street, Toronto, Ont. M3J 1P3, Canada |
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Abstract: | LacI? mutants obtained following 2 and 6 h of thymine deprivation were cloned and sequenced. The mutational spectra recovered were dissimilar. After 2 h of starvation the majority of mutations were base substitutions, largely G: C→C: G transversions. Frameshift mutations but not deletions were observed. In contrast, following 6 h of starvation, with the exception of the G: C→C: G transversion, all possible base substitutions were recovered. Moreover, several deletions but no frameshift events were observed. The differences in the mutational spectra recovered after two periods of thymine deprivation highlight the role of altered nucleotide pools and the potential influence of DNA replication mechanisms. |
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Keywords: | Thymine deprivation LacI? mutants Escherichia coli lacI gene |
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