| 蚕豆叶肉原生质体电融合的研究 |
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| 引用本文： | 杨万年. 蚕豆叶肉原生质体电融合的研究[J]. 分子细胞生物学报, 1986, 0(3) |
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| 作者姓名： | 杨万年 |
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| 作者单位： | 武汉大学病毒学系 |
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| 摘 要： | 本文研究了蚕豆叶肉原生质体经透明质酸酶、核糖核酸酶、神经氨酸酶、碱性磷酸酶、胰蛋白酶、脂肪酶六种水解酶和SDS、Triton X-100、CTMAB三种表面活性剂以及秋水仙素、细胞松驰素B处理后的电融合过程。结果表明:胰蛋白酶处理后的原生质体融合率明显下降;碱性磷酸酶、脂肪酶以及核糖核酸酶、透明质酸酶、神经氨酸酶处理的原生质体电融合率均有不同程度的上升。Triton X-100和CTMAB促进原生质体的电融合,但较高浓度(0.01%)的SDS起抑制作用。秋水仙素和细胞松驰素B处理的原生质体其电融合率有较大幅度的增高。
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STUDIES ON THE ELECTRIC-FUSION OF MESOPHYLL PROTOPLASTS OF VICIA FABA |
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| Yang Wan-nian. STUDIES ON THE ELECTRIC-FUSION OF MESOPHYLL PROTOPLASTS OF VICIA FABA[J]. Journal of Molecular Cell Biology, 1986, 0(3) |
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| Authors: | Yang Wan-nian |
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| Abstract: | The electric-fusion of mesophyll proto-plasts of Vicia faba with pretreatment of hya-luronidase,ribonuclease,neuraminidase,alka-line phosphatase,tripsin,lipase and sodiumdodecyl sulfate(SDS),Triton X-100.cetyl-trimethylammonium bromide(CTMAB)andcolchicine,cytochalasin B was studied.Theresults showed that the fusion index wasdecreased with pretreatment of tripsin andincreased with pretreatment of all other hy-drolases.Triton X-100 and CTMAB increasedthe fusion index but higher concentration(0.01%)SDS decreased.Pretreatment of col-chicine and cytochalasin B on the protoplastsmarkedly enhanced the fusion index. |
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| Keywords: | electric-fusion.protoplasts.hydrolases and detergents.colchicine and cytochalasin B. |
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