Control of dehydrodiferulate cross-linking in pectins from sugar-beet tissues |
| |
Authors: | Baydoun Elias A -H Pavlencheva Natalie Cumming Carol M Waldron Keith W Brett Christopher T |
| |
Institution: | Department of Biology, American University of Beirut, Beirut, Lebanon. |
| |
Abstract: | Pectins were extracted from roots, petioles and leaves of sugar beet, and cross-linked using hydrogen peroxide and peroxidase. The effects on dehydrodiferulate formation were monitored by HPLC and TLC. Dehydrodimers were formed in different proportions to those found in vivo. There was a net loss of around 50% of the phenolic groups (monomers plus dimers) during dimerisation. Gel filtration showed that root and petiole pectin, but not leaf pectin, increased in molecular weight during cross-linking. The effects of varying the cross-linking conditions were investigated, and it was found that hydrogen peroxide concentration was the most important factor in controlling both the type and amount of dehydrodiferulate formed. |
| |
Keywords: | Beta vulgaris L Chenopodaciaceae Sugar beet Pectin Cross-linking Ferulate Dehydrodiferulate Hydrogen peroxide Peroxidase |
本文献已被 ScienceDirect PubMed 等数据库收录! |