Glycogen-binding protein components of rat tissues |
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| Authors: | K Satoh K Sato |
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| Institution: | Second Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-Cho, Hirosaki 036, Japan |
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| Abstract: | A glycogen-adipoyldihydrazide-Sepharose 4B column has been prepared for the analysis of glycogen-binding protein components of rat tissues. Glycogen-metabolizing enzymes; glycogen synthase, phosphorylase, branching enzyme, and debranching enzyme of skeletal muscle and liver have been adsorbed to the column, while those of brain showed very low affinities to it. On SDS gel electrophoresis of the glycogen-binding protein fractions, at least five and nine additional protein components have been detected in skeletal muscle and liver, respectively. |
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| Keywords: | Suc succinyl pNA p-nitroanilide Ac acetyl t-Boc tert-butyloxycarbonyl Z benzyloxycarbonyl DCC N N′-dicyclohexylcarbodiimide HOBt 1-hydroxybenzotriazole DPPA diphenylphosphorazidate PCA perchloric acid Plg plasminogen |
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