乳杆菌DM9811发酵液提取物中RNA组分对水泡性口炎病毒抑制作用研究

目的通过乳杆菌DM9811发酵液提取物中RNA组分对水泡性口炎病毒(VSV)抑制作用研究,探讨其在抵抗肠道病毒性感染性疾病方面的作用。方法应用50%细胞感染计量法(TCID50)、免疫荧光法、ELISA和MTT法探讨不同浓度的RNA组分对VSV的抑制作用。结果 RNA组分200μg/mL浓度时与对照比较,RNA组分具有竞争性抑制VSV感染作用,细胞存活率为(90.9±3.67)%,并具有阻断VSV侵入细胞作用,细胞存活率为(96.6±1.47)%。但RNA组分对病毒生物合成的抑制作用不明显。此外RNA组分具有诱导BALB/c小鼠脾细胞产生IFN-α作用,并呈现一定的剂量依赖关系。结论乳杆菌DM9811发酵液提取物中RNA组分对VSV具有明显抑制作用。

Antiviral activity of the RNA from Lactobacillus DM9811 culture filtrate

Objective To study the antiviral activity of the RNA from Lactobacillus DM9811 culture filtrate,and explore the RNA antiviral mechanisms. Method Different experimental procedures（according to viral infection course） to assay the potential antiviral activity of RNA was used.Cell survival and viral inhibition were determined by antiviral assay（MTT and TCID50） and confirmed by immunofluorescence. Result With the concentration of the RNA from Lactobacillus DM9811 culture filtrate from 10 μg/mL to 200 μg/mL,there were no cell toxicity effects.Its possible mechanisms of antiviral activity included：（1） competitively inhibiting the VSV infecting the WI-38 cells（cell survival rate 90.9%±3.67%）;（2） interfering virus attachment or entry in to the cells,perhaps by steric hindrance（cell survival rate 96.6%±1.47%）;（3） inducing the BALB/c mouse splenocytes to produce IFN-α to modulate the virus infection with dose dependentce. Conclusion The RNA from Lactobacillus DM9811 culture filtrate shows active antiviral activity.