Expression of SHH signaling pathway components in the developing human lung |
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Authors: | Mingfeng Zhang Hong Wang Hongqi Teng Jueping Shi Yanding Zhang |
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Institution: | (1) Fujian Key Laboratory of Developmental Biology and Neurobiology, College of Life Sciences, Fujian Normal University, Qishan Campus, Fuzhou, 350108, Fujian, People’s Republic of China; |
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Abstract: | The Sonic hedgehog (Shh) cascade is crucial for the patterning of the early lung morphogenesis in mice, but its role in the
developing human lung remains to be determined. In the present study, the expression patterns of SHH signaling pathway components,
including SHH, PTCH1, SMO, GLI1, GLI2 and GLI3 were examined by in situ hybridization and immunohistochemistry, and compared with the equivalent patterns in mice. Our results
showed that, as in mice, SHH was expressed in the epithelium of the developing human lung. However, SHH receptors (PTCH1 and SMO) and SHH signaling effectors (GLI1-3) were strongly detected in the human lung epithelium, but weakly in the mesenchyme, slightly different from their expressions
in mice. Furthermore, the expression levels of SHH signaling pathway genes in human lung, but not that of GLI1, were subsequently downregulated at the canalicular stage evaluated by real-time PCR, coincident with a decline in the developing
murine lung. In conclusion, in spite of slight differences, the considerable similarities of gene expression in human and
mice suggest that conserved molecular networks regulate mammalian lung development. |
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