Isolation and characterization of a thermophilic Bacillus sp. JF8 capable of degrading polychlorinated biphenyls and naphthalene |
| |
Authors: | Minoru Shimura Gouri Mukerjee-Dhar Kazuhide Kimbara Hiroko Nagato Hohzoh Kiyohara Takashi Hatta |
| |
Institution: | Environmental Biotechnology Laboratory, Railway Technical Research Institute, Tokyo, Japan. mino@rtri.or.jp |
| |
Abstract: | Bacillus sp. strain JF8, which was isolated from compost, utilizes naphthalene and biphenyl as carbon sources at 60 degrees C. Biphenyl grown cells of strain JF8 barely degraded naphthalene while naphthalene grown cells did not degrade p-chlorobiphenyl, suggesting the existince of two independent degradation pathways. Isolation of JF8N, a mutant strain which can not utilize biphenyl as a carbon source while retaining the ability to utilize naphthalene, supports this hypothesis. Biphenyl grown cells of strain JF8 can degrade several polychlorinated biphenyl congeners including tetra- and pentachlorobiphenyl. bph and nah probes from mesophilic organisms failed to hybridize to strain JF8 DNA. |
| |
Keywords: | Polychlorinated biphenyl Naphthalene Biodegradation Bacillus sp |
本文献已被 ScienceDirect 等数据库收录! |