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Molecular Cloning and Characterization of a Microsatellite Locus Found in an RAPD Marker of a Spider Mite, Panonychus Citri (Acari: Tetranychidae) |
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| Authors: | Mh. Osakabe Norihide Hinomoto Satoshi Toda Shinkichi Komazaki Koichi Goka |
| Affiliation: | (1) Crop Entomology Laboratory, Department of Plant Protection, National Agriculture Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan;(2) National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, 305-8634, Japan;(3) Persimmon and Grape Research Center, National Institute of Fruit Tree Science, Akitsu, Hiroshima, 729-2494, Japan;(4) National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-0053, Japan |
| Abstract: | Genetic markers were searched using PCR with 40 kinds of decanucleotide primers to investigate DNA polymorphism in Panonychuscitri. A region consisting of a variable number of CT tandem repeats (microsatellite) was found in a fragment amplified with the OPB10 primer. The microsatellite differed in size by ca. 100 bp among several P. citri populations screened and was derived from at least seven alleles. This region was characteristic of P. mori and P. osmanthi, but was lacking in P. ulmi. The flanking regions were highly conserved among these species. |
| Keywords: | Panonychus citri spider mite microsatellite RAPD PCR DNA polymorphism |
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