Cytoplasmic illuminations: In planta targeting of fluorescent proteins to cellular organelles |
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| Authors: | Chris Hawes Claude M. Saint-Jore Federica Brandizzi Huanquan Zheng Alexandra V. Andreeva Petra Boevink |
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| Affiliation: | (1) Research School of Biological and Molecular Sciences, Oxford Brookes University, OX3 OBP Oxford, UK;(2) Unit of Cell Biology, Scottish Crop Research Institute, Dundee |
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| Abstract: | Summary Use of the jellyfish green-fluorescent protein as an in vivo reporter is in the process of revolutionising plant cell biology. By fusing the protein to specific targeting peptides or to sequences of complete proteins, it is now possible to observe the location, structure, and dynamics of a number of intracellular organelles over extended periods of time. In this review we discuss the most recent developments and unexpected results originating from the targeting of this unique protein and its derivatives to elements of the cytoskeleton and to membrane-bounded organelles in a range of plant cell types.Dedicated to Professor Brian E. S. Gunning on the occasion of his 65th birthday |
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| Keywords: | Green-fluorescent protein Plant cell Organelle Targeting |
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