蛋鸡J亚群白血病病毒的分离鉴定及序列分析

通过接种鸡胚成纤维细胞((CEF)及特异性单抗的间接荧光抗体反应(IFA),从中国商品代蛋鸡群中首次分离到J亚群白血病病毒(ALV-J).对其env基因编码的氨基酸序列及3'-末端(3'-Ter)序列与国内外来源于白羽肉鸡的毒株作了比较分析.结果显示,这两株病毒的gp85基因编码的氨基酸序列与国外5个毒株同源性仅为83.4%～87.3%,与国内来源于白羽肉鸡的8株病毒同源性也仅为86.4%～89.6%.gp37基因编码的氨基酸序列与5个国外毒株同源性为91.8%～97.0%,与8个国内毒株同源性为93.9%～95.9%.另外,国内来源于白羽肉鸡的各毒株的3'-Ter序列在"E"区均有明显缺失,但本次分离的来源于蛋鸡群的毒株在"E"区没有缺失突变.与所列出的13株国内外毒株相比,这两个毒株在3'-Ter的缺失最少,较接近于原型株HPRS-103.显然这两株病毒的来源不同于国内白羽肉鸡.

The Identification and Sequence Analysis of ALV-J Isolated from Layers

Two Avian leukosis viruses of subgroup J(ALV-J)were isolated from layers by inoculating the sample into chicken embryo fibroblast(CEF)cells and by indirect fluorescent assay(IFA)with ALV-J specific monoclonal antibody JE-9.Sequence comparison indicated the gp85 identities were only in the ranges of 83.4%-87.3% compared with five international reference strains and 86.4%-89.6% compared with eight Chinese strains isolated from white meat-type chickens.The gp37 identities were in the range of 91.8-96.4% compared with the five international strains and 93.4-95.9% compared with the eight domestic strains.When compared with the above thirteen strains,two layers' isolates were more close to the prototype HPRS-103 and had less deletion in 3'-Ter than those strains.All strains isolated from white meat-type chickens in China had a deletion in the "E" region of 3'-Ter except these two isolates,suggesting these two ALV-J isolates from layers have different evolution origins from other Chinese isolates from white meat-type chickens.