新型球孢白僵菌孢子悬乳剂的高效杀蚜活性及其评价方法

用球孢白僵菌（Beauveria bassiana）SG8702的孢子悬乳剂与未剂型化孢子粉对桃蚜进行了杀蚜活性对比测定。孢子悬乳剂与孢子粉分别用水稀释成5个序列浓度，对甘蓝叶片上蚜虫进行相同时间的弥雾接种，前者孢子附着量分别为1.5～701.1个孢子/mm2 ，后者附着量分别为2.8～1005.9个孢子/mm2。蚜虫接种后置于23℃和12L：12D条件下饲养，定时观察8d。经时间—剂量—死亡率模拟分析，悬乳剂的剂量效应参数明显高于孢子粉，且杀蚜时间效应提前。用模型参数估计悬乳剂和孢子粉的LC50，接种后第4天分别为9.0和634个孢子/mm2，第7天为3.3和5.3个孢子/mm2。悬乳剂和孢子粉的LT50随叶面孢子附着量增大而下降，在100个孢子/mm2下为3.2d和4.5d。这表明孢子悬乳剂的杀蚜活性比未剂型化的孢子粉显著增强。作者讨论了杀虫微生物制剂评价的技术规范问题。

Evaluation for Enhanced Aphidicidal Activity of a Noval Emulsifiable Formulation of Beauveria bassiana Conidia

A noval emulsifiable formulation (EF) of Beauveria bassiana SG8702 containing 10(10) conidia/mL was prepared by suspending dry conidia in mineral oil-based liquid containing emulsifier, stabilizer and UV protectant (emulsion), and then bioassayed on newly merged adults of the green peach aphid, Myzus percisae, in parallel to the unformulated conidia (UC). For inoculation, five gradient concentrations of aqueous EF and UC suspensions (5 x 10(5) - 1 x 10(8) conidia/mL) were separately sprayed on to aphids on detached cabbage leaves (30 - 40 adults/leaf) using a Micro Ulva hand-held sprayer, generating EF dosages of 1.5, 6.3, 32.6, 87.6 and 701.1 conidia/mm2, and UC dosages of 2.8, 9.2, 52.7, 114.8 and 1005.9 conidia/mm2, respectively. All dosage treatments including controls for EF (100-fold dilution of the emulsion) and UC (water spray) were replicated three times (aphid colonies on three leaves), and maintained in Petri dishes at 23 degrees C and 12 : 12 (L: D) for 8-day daily records. Cadavers, whenever found, were moved into a moist chamber to facilitate fungal outgrowths for verification of B. bassiana infection. The resulting data fit very well to time-dose-mortality model with EF having a larger dose effect (1.05 +/- 0.10) than UC (0.82 +/- 0.11) and also faster time effect. The estimates of the dose- and time-effect parameters were then used to compute lethal concentrations (LC50) and time lengths (LT50). The estimates of LC50 with 95% confidence intervals for both EF and UC were 9.0 (6.9 - 11.6) and 634 (263 - 1532) conidia/mm2 on day 4 after spray, and 3.3 (2.4 - 4.6) and 5.3 (3.2 - 8.6) on day 7, apparently decreasing with increasing time lengths after spray. The estimates of LT50 for EF and UC were 4.9 d and 7.2 d at 5 conidia/mm2, 4.0 d and 5.6 d at 10 conidia/mm2, and 3.2 d and 4.5 d at 100 conidia/mm2, indicating a trend of decrease with increasing counts of conidia attached to the leaves. The results indicate that the emulsifiable formulation of B. bassiana conidia had greater aphidicidal activity than the unformulated conidia. Finally, problems with evaluation of mycoinsecticides and other microbial formulations are discussed and technical standards are recommended.