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Performance of a recombinant strain of<Emphasis Type="Italic"> Streptomyces lividans</Emphasis> for bioconversion of penicillin G to deacetoxycephalosporin G |
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| Authors: | Q?Gao J?M?Piret J?L?Adrio |
| Institution: | (1) Charles A. Dana Research Institute for Scientists Emeriti, Drew University, NJ 07940, Madison, USA,;(2) Department of Biology, Massachusetts Institute of Technology, MA 02139, Cambridge, USA,;(3) Department of Biology, Northeastern University, MA 02115, Boston, USA,;(4) Microbielle Genetik, Universitaet Tuebingen, Waldhaeuserstrasse 70/8, 72076, Tuebingen, Germany,;(5) , Puleva Biotech, 66 Camino de Purchil, 18004, Granada, Spain, |
| Abstract: | We examined the performance of Streptomyces lividans strain W25 containing a hybrid expandase (deacetoxycephalosporin C synthase; DAOCS) gene, obtained by in vivo recombination between the expandase genes of S. clavuligerus and Nocardia lactamdurans for resting-cell bioconversion of penicillin G to deacetoxycephalosporin G. Strain W25 carried out a much more effective level of bioconversion than the previously used strain, S. clavuligerus NP1. The two strains also differed in the concentrations of FeSO4 and α-ketoglutarate giving maximal activity. Whereas NP1 preferred 1.8 mM FeSO4 and 1.3 mM α-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO4 and 1.9 mM α-ketoglutarate. Electronic Publication |
| Keywords: | Antibiotics Bioconversions Deacetoxycephalosporin Expandase Directed evolution Penicillin |
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