Transformation of potato via Agrobacterium coated microparticle bombardment |
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Authors: | T. T. Nguyen P. J. Dix G. D. Nugent |
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Affiliation: | (1) Laboratory of Plant Breeding, Graduate School Experimental Plant Sciences, Wageningen University, POBox 386, 6700 AJ Wageningen, The Netherlands;(2) Present address: Monsanto Co., Missouri, U.S.A.;(3) Biotechnology Unit, CIAT, AA 6713 Cali, Colombia |
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Abstract: | The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm−3. |
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