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Sequential detection of antigens in Western blots with differently colored products
Authors:N Theisen  E S Lohoff  K von Figura  A Hasilik
Affiliation:Physiologisch-Chemisches Institut der Universität Münster, Waldeyerstrasse 15, D-4400 Münster, Federal Republic of Germany
Abstract:A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.
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