Sequential detection of antigens in Western blots with differently colored products |
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Authors: | N Theisen E S Lohoff K von Figura A Hasilik |
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Affiliation: | Physiologisch-Chemisches Institut der Universität Münster, Waldeyerstrasse 15, D-4400 Münster, Federal Republic of Germany |
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Abstract: | A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot. |
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