| 寡糖素对西洋参和人参愈伤组织培养的影响 |
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| 引用本文： | 周立刚,郑光植.寡糖素对西洋参和人参愈伤组织培养的影响[J].天然产物研究与开发,1992,4(1):16-19. |
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| 作者姓名： | 周立刚 郑光植 |
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| 作者单位： | 中国科学院昆明植物研究所，中国科学院昆明植物研究所，中国科学院昆明植物研究所，中国科学院昆明植物研究所 昆明 650204，昆明 650204，昆明 650204，昆明 650204 |
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| 摘 要： | 红花、人参和黑节草寡糖素(简称CO、GO和DO)分别加入到培养基中,均能影响西洋参和人参愈伤组织生长和皂甙的合成。CO、GO和DO促进西洋参愈伤组织皂甙合成的最适浓度分别为5ppm、15ppm和10ppm,皂甙产率分别为14.89mg/flask、11.24mg/flask和14.53mg/flask,均明显高于对照(8.22mg/flash).CO、GO和DO促进人参愈伤组织皂甙合成的最适浓度分别为5ppm、20ppm和5ppm,皂甙产率分别为11.79mg/flask、11.20mg/flask和10.48mg/flask,均明显高于对照(6.65mg/flask),它们在适度浓度下对人参愈伤组织的生长均有促进作用。
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| 关 键 词： | 寡糖素 西洋参 人参 愈伤组织 |
EFFECTS OF OLIGOSACCHARINS ON CALLUS CULTURE OF PANAX QUINQUEFOLIUM AND P.GINSENG |
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| Zhou Ligang,Zheng Guangzhi,Wang Shilin,Gan Fanyuan Kunming Institute of Botany,Academia Sinica,Kunming.EFFECTS OF OLIGOSACCHARINS ON CALLUS CULTURE OF PANAX QUINQUEFOLIUM AND P.GINSENG[J].Natural Product Research and Development,1992,4(1):16-19. |
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| Authors: | Zhou Ligang Zheng Guangzhi Wang Shilin Gan Fanyuan Kunming Institute of Botany Academia Sinica Kunming |
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| Institution: | Zhou Ligang,Zheng Guangzhi,Wang Shilin,Gan Fanyuan Kunming Institute of Botany,Academia Sinica,Kunming 650204 |
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| Abstract: | Cligosaccharins of Calpamus lincioris, Panax ginseng and Dendrobi- um candidum (abbreviated as CO, GO and Do separately)which were added into the media could effect the callus growth and saponin synthesis of P. quinque jolium and P. ginseng. The appropriate concentration of CO, GO and DO wre 5ppm, 15ppm and 10Ppm separately to stimulate saponin synthesis of P. quinque jolium callus which saponin yield wre 14.90mg/ flask, 11.24mg/flask and 14.53mg/flask separately, and saponin yield of the control was only 8.22mg/flask. The appropriate concentration of CO, GO and DO wre 5ppm 20ppm and 5ppm separately to stimulate synthesis of P. ginseng callus which saponin yields were 11.79mg/flask, 11.20mg/flask and 10.48mg/flask separately, and the control saponin yield was only 6.65mg/ flask. The oligosaccharins all stimulated the callus growth of P.gin eng in an appropriate concentration. |
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| Keywords: | Oligosaccharin Panax quinque jolium Panax ginseng Callus Saponin |
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