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| JEV-DNA实时荧光定量标准品的构建 | |
| 引用本文： | 周净,王云龙,李智涛,李玲玲. JEV-DNA实时荧光定量标准品的构建[J]. 生物技术通报, 2007, 0(4): 178-180,185 |
| 作者姓名： | 周净 王云龙 李智涛 李玲玲 |
| 作者单位： | 1. 河南师范大学,新乡,453007;郑州职业技术学院,郑州,450121 2. 郑州职业技术学院,郑州,450121 3. 河南省生物工程技术研究中心,郑州,450002 4. 河南师范大学,新乡,453007;河南省生物工程技术研究中心,郑州,450002 |
| 摘 要： | 利用TaqMan荧光定量PCR技术,建立JEV-DNA定量标准品的制备方法。通过处理JEV减毒活疫苗提取病毒RNA,进行RT-PCR扩增目的片段,与T载体连接,转化感受态细胞,T-A克隆,测序鉴定后定量。获得预期的重组质粒,建立的标准曲线有较大的线性范围。此法制备的重组质粒标准品可用于对病毒载量进行测定。 |
| 关 键 词： | 标准品 实时荧光定量PCR 乙型脑炎病毒 |
| 修稿时间： | 2007-01-29 |
Construction of Plasmid Standards for Real-time Fluorescence Quantitative PCR of JEV-DNA |
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| Zhou Jing,Wang Yunlong,Li Zhitao,Li Lingling. Construction of Plasmid Standards for Real-time Fluorescence Quantitative PCR of JEV-DNA[J]. Biotechnology Bulletin, 2007, 0(4): 178-180,185 | |
| Authors: | Zhou Jing Wang Yunlong Li Zhitao Li Lingling |
| Affiliation: | 1 Henan Normal University,Xinxiang 453007 ; 2Zhengzhou Technology College, Zhengzhou 450121 ; 3 Henan Bioteehnology Research Center,Zhengzhou 450002 |
| Abstract: | This experiment is to establish a method of preparation for the standard plasmids of JEV-DNA by using real-time fluorescence quantitative PCR.From live attenuated JE vaccine by RT-PCR,target genes were amplified then cloned into T vector,sequence identified,and quantify by real-time fluorescence quantitative PCR assay.The standard plasmids were gotten.Standard curve created by plasmid standards had wide detection range.It is viable that the plasmid standards can be used for virus loads detection. |
| Keywords: | Standard substance Real-time fluorescence quantitative PCR Japanese encephalitis virus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |