Molecular signatures of long‐lived proteins: autolytic cleavage adjacent to serine residues

The centre of the human lens, which is composed of proteins that were synthesized prior to birth, is an ideal model for the evaluation of long‐term protein stability and processes responsible for the degradation of macromolecules. By analysing the sequences of peptides present in human lens nuclei, characteristic features of intrinsic protein instability were determined. Prominent was the cleavage on the N‐terminal side of serine residues. Despite accounting for just 9% of the amino acid composition of crystallins, peptides with N‐terminal Ser represented one‐quarter of all peptides. Nonenzymatic cleavage at Ser could be reproduced by incubating peptides at elevated temperatures. Serine residues may thus represent susceptible sites for autolysis in polypeptides exposed to physiological conditions over a period of years. Once these sites are cleaved, other chemical processes result in progressive removal or ‘laddering’ of amino acid residues from newly exposed N‐ and C‐termini. As N‐terminal Ser peptides originated from several crystallins with unrelated sequences, this may represent a general feature of long‐lived proteins.