Heterologous expression and characterization of a novel halotolerant,thermostable, and alkali-stable GH6 endoglucanase from <Emphasis Type="Italic">Thermobifida halotolerans</Emphasis> |
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| Authors: | Yi-Rui Yin Feng Zhang Qing-Wen Hu Wen-Dong Xian Wael N Hozzein En-Min Zhou Hong Ming Guo-Xing Nie Wen-Jun Li |
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| Institution: | 1.Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University,Kunming,People’s Republic of China;2.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, and School of Life Sciences, Fujian Agriculture and Forestry University,Fuzhou,People’s Republic of China;3.State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University,Guangzhou,People’s Republic of China;4.Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,ürümqi,People’s Republic of China;5.Bioproducts Research Chair, College of Science,King Saud University,Riyadh,Kingdom of Saudi Arabia;6.College of Fisheries,Henan Normal University,Xinxiang,People’s Republic of China |
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| Abstract: | A novel endoglucanase gene was cloned from Thermobifida halotolerans YIM 90462T, designated as thcel6A for being a member of glycoside hydrolase family 6. The gene was 1332 bp long and encoded a 443-amino-acid protein with a molecular mass of 45.9 kDa. The purified recombinant endoglucanase had optimal activity at 55 °C and pH 8.5. Thcel6A showed high hydrolytic activities at 25–55 °C and retained 58 % of initial activity after incubation at 90 °C for 1 h. It retained more than 80 % of activity after incubation for 12 h at pH values from 4 to 12. Thcel6A displayed higher hydrolytic activities in 5–15 % NaCl (w/v) than at 0 % NaCl. Activity increased 2.5-fold after incubation with 20 % (w/v) NaCl at 37 °C for 10 min. These properties suggest that this novel endoglucanase has potential for specific industrial application. |
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