Interaction between crystalline proteins of two Bacillus thuringiensis strains against Spodoptera exigua |
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| Authors: | Edyta Konecka Jakub Baranek Adam Kaznowski Jadwiga Ziemnicka Kazimierz Ziemnicki |
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| Institution: | 1. Department of Microbiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61‐614 Poznań, Poland;2. Department of Biological Control, Institute of Plant Protection – National Research Institute, W?. W?gorka 20, 60‐318 Poznań, Poland;3. Department of Physiology and Animal Development Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61‐614 Poznań, Poland |
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| Abstract: | The aim of the present article was to evaluate potential synergism between crystalline proteins produced by two Bacillus thuringiensis Berliner strains, MPU B6 and MPU B9, against beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae). Protein inclusions of bacterial strains were isolated from a spore‐crystal mixture. We estimated the 50% lethal concentration (LC50) of crystals for S. exigua larvae. Insecticidal activity of MPU B6 and MPU B9 individual crystal preparations against caterpillars were determined and compared with the commercial pesticide Foray. Protein crystals of MPU B9 had the highest toxicity against S. exigua. The proteins were approximately 25× more toxic than Foray. Insecticidal activity of protein crystals of MPU B6 isolate was approximately 2.5× higher than that of Foray. A mixture of crystals suspensions of both isolates MPU B6/MPU B9 had an additive effect on S. exigua caterpillars. The high insecticidal potency of B. thuringiensis MPU B9 crystals against S. exigua predisposes the strain for additional studies on production of a new effective preparation against pest insects. |
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| Keywords: | bacterial toxins insecticidal activity plant protection beet armyworm Lepidoptera Noctuidae additive effect synergism |
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