Nucleus‐encoded mRNAs for Chloroplast Proteins GapA,PetA, and PsbO are Trans‐spliced in the Flagellate Euglena gracilis Irrespective of Light and Plastid Function |
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| Authors: | Bianka Mateášiková‐Kováčová Matej Vesteg Hana Drahovská Kristína Záhonová Rostislav Vacula Juraj Krajčovič |
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| Affiliation: | 1. Department of Biochemistry, Faculty of Natural Sciences, Comenius University, , Mlynská dolina, 842 2. 15 Bratislava, Slovakia;3. Institute of Cell Biology and Biotechnology, Faculty of Natural Sciences, Comenius University, , Mlynská dolina, 842 4. Department of Genetics, Faculty of Natural Sciences, Comenius University, , Mlynská dolina, 842 5. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, , Mlynská dolina, 842 |
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| Abstract: | Euglena gracilis is a fresh‐water flagellate possessing secondary chloroplasts of green algal origin. In contrast with organisms possessing primary plastids, mRNA levels of nucleus‐encoded genes for chloroplast proteins in E. gracilis depend on neither light nor plastid function. However, it remains unknown, if all these mRNAs are trans‐spliced and possess spliced leader sequence at the 5′‐end and if trans‐splicing depends on light or functional plastids. This study revealed that polyadenylated mRNAs encoding the chloroplast proteins glyceraldehyde‐3‐phosphate dehydrogenase (GapA), cytochrome f (PetA), and subunit O of photosystem II (PsbO) are trans‐spliced irrespective of light or plastid function. |
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| Keywords: | bleaching secondary endosymbiosis SL‐RNA trans‐splicing white mutant |
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