Leishmania donovani: Metabolite mapping of promastigotes using proton nuclear magnetic resonance spectroscopy |
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| Authors: | U.K. Singha Vinod Bhakuni Vahab Ali Raja Roy |
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| Affiliation: | (1) Division of Membrane Biology, Central Drug Research Institute, Lucknow, India;(2) Medicinal Chemistry (RSIC), Central Drug Research Institute, Lucknow, India;(3) Division of Membrane Biology, Central Drug Research Institute, Lucknow Chattar Manzil, Post Box No. 173, 226 001 Lucknow, India |
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| Abstract: | Proton nuclear magnetic resonance spectroscopy was used for studying the intracellular metabolite profile of promastigotes of Leishmania donovani. The major intracellular metabolites observed in the promastigotes were acetate, alanine, succinate, glycine,  -glycerophosphorylcholine, acetoacetate, arginine and ethanol. A comparative study of the intracellular metabolite profile of promastigotes of different strains of L. donovani showed that, all the major intracellular metabolites were present in promastigotes of different strains. A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation. |
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| Keywords: | Leishmania donovani promastigotes metabolites nuclear magnetic resonance (NMR) perchloric acid extract (PCA) strain differentiation |
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