Immobilization of invertase on rice husk using polyethylenimine |
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Authors: | D'Souza S F Godbole S S |
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Institution: | Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. sfdsouza@apsara.barc.ernet.in |
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Abstract: | Washed and dried rice husk was coated with 2% polyethylenimine (PEI). Invertase was immobilized on this support through adsorption followed by cross-linking with 2% glutaraldehyde. Immobilized enzyme was reused for the hydrolysis of sucrose without loss in activity. This approach may serve as a simple technique in the future for the covalent immobilization of enzymes on lignocellulosic supports. |
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