A Pseudomonas aeruginosa biosensor responds to exposure to ultraviolet radiation |
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| Authors: | M O Elasri R V Miller |
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| Institution: | (1) Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Ok-74078, USA e-mail: rum67@okway.okstate.edu Tel.: +1-405-7446243 Fax: +1-405-7446790, US |
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| Abstract: | We fused the Pseudomonas aeruginosa recA promoter to a promoterless Vibrio fisherilux operon. This recA–lux fusion (pMOE15) was introduced into wild-type P. aeruginosa strain FRD1 and recA expression was monitored by measuring 490-nm light production. The RM4440 strain responded to increasing doses of ultraviolet
radiation by an increase in its bioluminescence. RM4440 has the potential to be useful as a biosensor for the presence of
DNA-damaging agents in the environment.
Received: 18 February 1998 / Received revision: 18 June 1998 / Accepted: 27 June 1998 |
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