Effects of hepatocyte growth factor (HGF) and activin a on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture |
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| Authors: | Miho Furue Tetsuji Okamoto Hiroyuki Hayashi J. Denry Sato Makoto Asashima Shigeru Saito |
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| Affiliation: | (1) Department of Oral Biochemistry, Kanagawa Dental College, 82 Inaoka-cho, 238-8580 Yokosuka City, Kanagawa, Japan;(2) Department of Oral and Maxillofacial Surgery I, Hiroshima University School of Dentistry, 1-2-3 Kasumi, 734-0037 Hiroshima, Japan;(3) Department of Oral Histochemistry, Kanagawa Dental College, USA;(4) Adirondack Biomedical Res. Inst., Lake Placid, 12946 New York;(5) Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153-0041 Tokyo, Japan |
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| Abstract: | Summary To study the mechanisms of morphogenesis in salivary gland regeneration, we have established the RSMG-1 cell line derived from submandibular gland (SMG) of 10-wk-old Wistar female rats in serum-free culture. Our finding that RSMG-1 cells originated from duct cells was based on morphology and immunohistochemical results. In three-dimensional serum-free collagen gel culture, HGF induced branching morphogenesis of RSMG-1 cells. Histological examination revealed that HGF-induced branching structure exhibited well-formed lumina. This morphology closely resembles that found in vivo. The cells also expressed activin A. Exogenously added activin A at a high concentration reduced HGF-induced branching morphogenesis. These findings suggest that the morphogenesis of the salivary gland is modulated by HGF and activin A. Our results show that the RSMG-1 cell line may be useful in studies of salivary gland regeneration. |
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| Keywords: | salivary gland HGF activin branching morphogenesis |
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