Proteomic Analysis of the Response of Liangyoupeijiu (Super High-Yield Hybrid Rice) Seedlings to Cold Stress |
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作者姓名: | Ping-Fang Yang Xiao-Juan Li Yu Liang Yu-Xiang Jing Shi-Hua Shen Ting-Yun Kuang |
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作者单位: | [1]Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China [2]Graduate College of the Chinese Academy of Sciences, Beijing 100039, China |
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基金项目: | Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-SW-307) and the Knowledge Innovation Program of the Institute of Botany, the Chinese Academy of Sciences. |
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摘 要: | Liangyoupeijiu is a super high-yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being widely cultivated. We subjected Liangyoupeijiu seedlings to 4 ℃ cold treatment, then extracted the leaf proteins. After 2-D gel electrophoresis separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, a series of differentially displayed proteins were identified. Some metabolism-associated proteins were found among the downregulated proteins, such as carbamoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase. The upregulated proteins included both stress-resistance proteins such as nucleoside diphosphate kinase I and proteins that are negative for rice growth, such as FtsH-like protein, plastid fusion and/or translocation factor (Pftf) and actin. Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure.
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关 键 词: | 杂交水稻 树苗 寒冷环境 漂白亚麻纤维卷 |
收稿时间: | 2005-08-12 |
修稿时间: | 2005-08-122005-11-08 |
Proteomic Analysis of the Response of Liangyoupeijiu (Super High-Yield Hybrid Rice) Seedlings to Cold Stress |
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Authors: | Ping-Fang Yang Xiao-Juan Li Yu Liang Yu-Xiang Jing Shi-Hua Shen Ting-Yun Kuang |
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Institution: | Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences;, Beijing 100093, China;Graduate College of the Chinese Academy of Sciences;, Beijing 100039, China |
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Abstract: | Liangyoupeijiu is a super high‐yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being widely cultivated. We subjected Liangyoupeijiu seedlings to 4°C cold treatment, then extracted the leaf proteins. After 2‐D gel electrophoresis separation and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry analysis, a series of differentially displayed proteins were identified. Some metabolism‐associated proteins were found among the downregulated proteins, such as carbamoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3‐phosphate dehydrogenase. The upregulated proteins included both stress‐resistance proteins such as nucleoside diphosphate kinase I and proteins that are negative for rice growth, such as FtsH‐like protein, plastid fusion and/or translocation factor (Pftf) and actin. Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure. (Managing editor: Li‐Hui Zhao) |
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Keywords: | cold stress Oryza sativa proteome super high-yield hybrid rice |
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