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Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana |
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| Authors: | Hong Young-Ho Lee Dong-Woo Lee Sang-Jae Choe Eun-Ah Kim Seong-Bo Lee Yoon-Hee Cheigh Chan-Ick Pyun Yu-Ryang |
| Institution: | (1) Department of Biotechnology, Yonsei University, Seoul, 120-749, Korea;(2) Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA;(3) Department of Human Genetics, Emory University, Atlanta, GA 30322, USA;(4) R&D Center of Sugar Flour & Edible Oil Planning, CJ Corp., Seoul, 100-802, Korea;(5) Department of Food Science and Technology, Ohio State University, Columbus, OH 43210, USA |
| Abstract: | Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t 1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h. |
| Keywords: | l-Arabinose isomerase" target="_blank">l-Arabinose isomerase Esherichia coli Immobilization d-Tagatose" target="_blank">d-Tagatose Thermotoga neapolitana |
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