Detection of bovine αs1-casein genomic variants using the allele-specific polymerase chain reaction |
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| Authors: | V A DAVID A H DEUTCH |
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| Institution: | BioMolecular Research Department, W. R. Grace & Co.-Conn., Columbia, Maryland 21044. |
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| Abstract: | A method was developed to distinguish between genotypic variants B and C of bovine alpha s1-casein, using the allele-specific polymerase chain reaction (ASPCR). The alpha s1-casein genotype determined for 17 Jersey cows by the ASPCR method was confirmed by typing the alpha s1-casein milk proteins on isoelectric focusing gels. Using the ASPCR method described, rapid analysis of the alpha s1-casein genotype of bulls is now possible. In addition, kappa-casein genotypes can be determined from the same PCR reaction. |
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| Keywords: | bovine dairy cattle αs1-casein allele-specific PCR allelic-variation genetic polymorphism K-casein |
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