IDENTIFICATION AND DNA SEQUENCE OF A NEW H+-ATPase IN THE UNICELLULAR GREEN ALGA CHLAMYDOMONAS REINHARDTII (CHLOROPHYCEAE) |
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Authors: | A Malcolm Campbell Alison J Coble Lindsay D Cohen Toh Hean Ch'Ng Kristin M Russo Elizabeth M Long† E Virginia Armbrust¶ |
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Institution: | Department of Biology, Davidson College, Davidson, North Carolina 28036;Molecular and Cell Biology, University of Washington, Seattle, Washington 98195;Marine Molecular Biotechnology Laboratory, School of Oceanography, Box 357940, University of Washington, Seattle, Washington 98195 |
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Abstract: | Insertional mutagenesis was used to identify genes involved in mating and/or zygote formation in the unicellular green alga Chlamydomonas reinhardtii Dangeard. Approximately 800 insertionally mutagenized transformants were examined, and a single nonagglutinating mutant was identified. Plasmid rescue was used to clone a genomic fragment containing transforming DNA. This fragment was then used to identify the wild-type copy of the gene disrupted during mutagenesis. The wild-type gene is transcribed during all stages of the life cycle and, based on sequence similarity, encodes a P2-type proton transporting ATPase. The gene is referred to as Pmh1 for plasma membrane H + -ATPase. PMH1 displays the greatest sequence similarity to ATPases from two parasitic flagellates and a raphidophytic alga but not to the ATPase from a closely related green alga. We propose that PMH1 represents a distinct H + -ATPase isoform expressed in flagellates. |
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Keywords: | Chlamydomonas DNA sequence flagella H+-ATPase mutagenesis |
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