Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants |
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Authors: | Setsuko Komatsu Guangxiao Yang Monowar Khan Haruko Onodera Seiichi Toki Masayuki Yamaguchi |
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Institution: | (1) National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba 305-8518, Japan;(2) National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan;(3) National Agricultural Research Center for Tohoku Region, Akita 014-0102, Japan |
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Abstract: | Calcium is a ubiquitous signaling molecule and changes in cytosolic calcium concentration are involved in plant responses
to various stimuli. The rice calcium-dependent protein kinase 13 (CDPK13) and calreticulin interacting protein 1 (CRTintP1)
have previously been reported to be involved in cold stress response in rice. In this study, rice lines transformed with sense
CDPK13 or CRTintP1 constructs were produced and used to investigate the function of these proteins. When the plants were incubated
at 5°C for 3 days, leaf blades of both the sense transgenic and vector control rice plants became wilted and curled. When
the plants were transferred back to non-stress conditions after cold treatment, the leaf blades died, but the sheaths remained
green in the sense transgenic rice plants. Expression of CDPK13 or CRTintP1 was further examined in several rice varieties
including cold-tolerant rice varieties. Accumulation of these proteins in the cold-tolerant rice variety was higher than that
in rice varieties that are intermediate in their cold tolerance. To examine whether over-expression of CDPK13 and CRTintP1
would have any effect on the proteins or not, sense transgenic rice plants were analyzed using proteomics. The 2D-PAGE profiles
of proteins from the vector control were compared with those of the sense transgenic rice plants. Two of the proteins that
differed between these lines were calreticulins. The results suggest that CDPK13, calreticulin and CRTintP1 might be important
signaling components for response to cold stress in rice. |
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Keywords: | Calreticulin CDPK13 CRTintP1 Cold Rice |
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