Effect of lead acetate on Sertoli cell lactate production and protein synthesis in vitro |
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| Authors: | Layla I Batarseh Michael J Welsh Michael J Brabec |
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| Institution: | (1) Department of Environmental and Industrial Health School of Public Health, The University of Michigan, Ann Arbor, Michigan;(2) Department of Anatomy and Cell Biology, The University of Michigan, Ann Arbor, Michigan;(3) Department of Chemistry, Eastern Michigan University, 48197 Ypsilanti, Michigan |
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| Abstract: | The effects of lead acetate on protein synthesis and lactate production by cultures of rat Sertoli cells in vitro were studied. Sertoli cell cultures prepared from 20 day old Sprague-Dawley rats were exposed to 0.01, 0.05 and 0.10 mM lead acetate. Lactate production was significantly elevated by all concentrations of lead after 3, 6, 9 and 12 hours of exposure. Protein biosynthesis as measured by 3H]-leucine incorporation was significantly depressed by 0.05 and 0.10 mM lead acetate after 2 hours of exposure. These results support the hypothesis that lead acetate may inhibit spermatogenesis by a disturbance of the metabolic activities of the Sertoli cells.Abbreviations MEM
minimal essential medium
- TCA
trichloroacetic acid |
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| Keywords: | lactate lead Sertoli cell spermatogenesis rat |
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