牛体内,外受精胚胎玻璃化冷冻保存技术的研究初报

利用３种培养液即输卵管合成液（ＳＯＦ）、ＴＣＭ１９９和ＣＲｌａａ对牛体外受精后的卵母细胞进行培养，结果卵裂率分别达８５％、６７％和７２％，囊胚发育率分别为３７％、２１％和３０％。对所获得的囊胚利用ＥＦＳ玻璃化溶液进行冷冻保存。在１０％ＥＧ中预处理５ｍｉｎ后再移入ＥＦＳ４０平衡３０ｓ二步法冷冻保存的胚胎，其１解冻后继续发育率高达８６％，与对照组９１％相比无显性差异（Ｐ＞０．０５）。而ＥＦＳ３０二步

The cryopreservation of bovine embryos derived from in vitro fertilization or in vivo fertilization by vitrification

Bovine oocytes from in vitro fertilization (IVF) were cultured in three kinds of mediums(SOF,TCM199 and CR1aa)in this experiment.The developmental rate of the embryos was 85%,67% and 72% respectively.The rate of the embryos developing to blastocysts was 37%,21% and 30%.The blastocysts were cryopreserved in EFS vitrification solutions.The embryos were pretreated in 10%EG for 5 min, then were equilibrated in EFS 40 for 30 s before vitrification(two-step method).The developmental rate after thawed was 86% which was not significantly different from the control(91%)(P>0.05).But when EFS 40 was replaced by EFS 30,the developmental rate was only 43%.The result was significantly different from the control(P< 0.01 ).The developmental rate of early blastocysts derived from in vivo fertilization cryopreserved by one-step method or two-step method in EFS 40 was 83% and 95% respectively which were not significantly different from the control(97%)(P>0.05).