1H, 15N and 13C assignments of yellow fluorescent protein (YFP) Venus |
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| Authors: | Shang-Te Danny Hsu Caroline Behrens Lisa D. Cabrita Christopher M. Dobson |
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| Affiliation: | (1) Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK |
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| Abstract: | We present here the backbone and side-chain NMR assignments of YFP Venus, a 238-residue protein that emits yellow fluorescence in its native state. Venus is a variant of the green fluorescent protein (GFP), which has improved chromophore maturation and brightness, and the photochemistry and photophysics of which are insensitive to experimental conditions, such as the pH value and buffer content, making it a favourable biomarker. |
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| Keywords: | YFP GFP Selective labelling Deuteration Methyl NMR |
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