Effects of Continuous Thermophilic Composting (CTC) on Bacterial Community in the Active Composting Process |
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Authors: | Yong Xiao Guang-Ming Zeng Zhao-Hui Yang Yan-He Ma Cui Huang Wen-Jun Shi Zheng-Yong Xu Jing Huang Chang-Zheng Fan |
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Institution: | (1) College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China;(2) Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, China;(3) Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China;(4) Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, China |
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Abstract: | The method of continuous thermophilic composting (CTC) remarkably shortened the active composting cycle and enhanced the compost
stability. Effects of CTC on the quantities of bacteria, with a comparison to the traditional composting (TC) method, were
explored by plate count with incubation at 30, 40 and 50°C, respectively, and by quantitative PCR targeting the universal
bacterial 16S rRNA genes and the Bacillus 16S rRNA genes. The comparison of cultivatable or uncultivatable bacterial numbers indicated that CTC might have increased
the biomass of bacteria, especially Bacillus spp., during the composting. Denaturing gradient gel electrophoresis (DGGE) analysis was employed to investigate the effects
of CTC on bacterial diversity, and a community dominated by fewer species was detected in a typical CTC run. The analysis
of sequence and phylogeny based on DGGE indicated that the continuously high temperature had changed the structure of bacterial
community and strengthened the mainstay role of the thermophilic and spore-forming Bacillus spp. in CTC run. |
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