Ultrafiltrative separation of rhamnolipid from culture medium |
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Authors: | Witek-Krowiak Anna Witek Joanna Gruszczyńska Anna Szafran Roman G Ko?lecki Tomasz Modelski Szymon |
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Institution: | (1) Department of Chemistry, Wrocław University of Technology, Norwida 4/6, 50-373 Wroclaw, Poland;(2) Institute of Environment Protection Engineering, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland |
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Abstract: | Classic methods of biosurfactant separation are difficult and require large amounts of organic solvents, thus generate high
amounts of waste. This work presents and discusses in detail an original procedure to separate rhamnolipid from fermentation
broth using high performance membrane techniques. Due to the unique properties of surface active agents, such as capability
of forming aggregates above the critical micelle concentration, it is possible to easily purify the biosurfactant with high
efficacy using inexpensive and commonly used membranes. In this article, two-stage ultrafiltration is proposed as a method
for separating and purifying rhamnolipid from the culture medium. The obtained purified rhamnolipid solution was capable of
reducing surface tension of water down to 28.6 mN/m at critical micelle concentration of 40 mg/l. Separation of rhamnolipid
was confirmed by HPLC; three types of rhamnolipids were identified (RL1, RL2, RL4), with considerable predominance of RL2. |
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