An apparatus for pulse and pulse-chase experiments with 14CO2 on attached leaves with known, steady-state rates of photosynthesis |
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Authors: | D. ALAN JONES ALISON M. SMITH HAROLD W. WOOLHOUSE |
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Affiliation: | ARC Research Group on Photosynthesis, John Innes Institute, Colney Lane, Norwich, Norfolk NR4 7UH |
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Abstract: | Abstract. An apparatus is described to carry out pulse and pulse-chase experiments with 14CO2 on intact, attached leaves with known, steady-state rates of photosynthesis under defined conditions of temperature, vapour pressure deficit and photon flux density. Data are presented which show that the pattern of distribution of 14C between compounds in extracts of such leaves is a true reflection of the pathways of photosynthetic carbon metabolism in the leaf during steady-state photosynthesis. |
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Keywords: | steady-state photosynthesis gas-exchange measurement 14CO2 pulse-chase |
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