Semiautomated preparation of DNA templates for large-scale sequencing projects |
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| Authors: | V Smith C M Brown A T Bankier B G Barrell |
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| Affiliation: | Medical Research Council Laboratory of Molecular Biology, Cambridge. |
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| Abstract: | The rate limiting step in a large-scale sequencing project is the generation of single-stranded DNA templates. We describe a fast, semiautomated procedure, using 96-well microtitre plates, in which 192 templates can be readily prepared in 1 day. The technique can be carried out manually or can be semiautomated using a robot pipetting device. We also provide evidence for the reliability and applicability of this method to a large-scale sequencing project. |
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