Distribution of legume arabinogalactan protein-extensin (AGPE) glycoproteins in symbiotically defective pea mutants with abnormal infection threads |
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Authors: | A V Tsyganova V E Tsyganov K C Findlay A Y Borisov I A Tikhonovich N J Brewin |
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Institution: | 1. All-Russia Research Institute for Agricultural Microbiology, St. Petersburg, Russia 2. John Innes Centre, Norwich, UK
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Abstract: | The interface between the host cell and the microsymbiont is an important zone for development and differentiation during
consecutive stages of Rhizobium-legume symbiosis. Legume root nodule extensins, otherwise known as arabinogalactan protein-extensins
(AGPEs) are abundant components of infection thread matrix. We have characterized the origin and distribution of these glycoproteins
at the symbiotic interface of root nodules of symbiotically defective mutants of pea (Pisum sativum L.) by using immunogold localization with MAC265 an anti-AGPE monoclonal antibody. For mutants with defective growth of infection
threads, the AGPE epitope was abundant in the extracellular matrix surrounding infected host cells in the central infected
tissue of the nodule, as well as in the lumen of Rhizobiuminduced infection threads. This seems to indicate a mistargeting
of AGPE as a consequence of abnormal growth of the infection threads. Furthermore, mutants in the gene sym33 showed reduced labeling with MAC265 and, in some infection threads and droplets, the label was completely absent, a phenomenon
that is not observed in wild-type nodules. This suggests an alteration in the composition of the infection thread matrix for
sym33 mutants, which may be correlated to the absence of endocytosis of rhizobia into the host cytoplasm. |
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