Genetic evidence of <Emphasis Type="Italic">Ranavirus</Emphasis> in toe clips: an alternative to lethal sampling methods |
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Authors: | V St-Amour D Lesbarrères |
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Institution: | (1) Biology Department, Laurentian University, Sudbury, ON, Canada, P3E 2C6 |
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Abstract: | Amphibian populations have been undergoing declines on a global scale. Among the many threats to these populations are emergent
infectious diseases (EIDs). The Ranavirus in particular has been found within many declining amphibian populations. Although non-lethal sampling methods exist for
some amphibian groups, such as salamanders, the anurans are traditionally tested using a lethal method. By comparing traditional
liver samples and a new non-lethal method of toe clipping we prove that the Ranavirus can also be determined in frogs using a non-lethal method, a much needed tool in threatened populations. This method will
allow for ranaviral detection without further impacting declining populations, and can further be used for other research
questions. |
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Keywords: | Ranavirus Non-invasive method Amphibian declines Rana clamitans |
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