Enzymic properties of intestinal aminopeptidase P: a new continuous assay |
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| Authors: | J Lasch R Koelsch T Steinmetzer U Neumann H U Demuth |
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| Affiliation: | Institute of Biochemistry, Medical Faculty, Martin-Luther-University Halle, GDR. |
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| Abstract: | A continuous photometric assay of aminopeptidase P activity was developed which is based on a coupled enzymic assay with the substrate Gly-Pro-Pro-pNA and DPP IV as auxiliary enzyme. This assay was used to evaluate the kinetic parameters and inhibitory profile of intestinal brush border aminopeptidase P. |
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