The role of calcium in depigmentation of iris epithelial cells during cell-type conversion |
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Authors: | Leslie Patmore Tuneo Yamada |
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Institution: | Swiss Institute for Experimental Cancer Research, CH-1066 Epalinges, Switzerland |
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Abstract: | During the conversion of newt iris epithelial cells into lens cells, melanosomes disappear from the cytoplasm. In this “depigmentation,” exocytosis of melanosomes is involved. The role of Ca2+ in this process has been the subject of this work. The intracellular Ca2+ concentration of cultured iris epithelial cells was increased by three methods: microinjection of 10?3, M CaCl2 into the cytoplasm, fusion of phospholipid vesicles containing 10?3, M CaCl2 with the cell membrane, and exposure to the calcium ionophore A23187. Each of these treatments caused an increase in the release of melanosomes. Further experiments suggest that cAMP stimulates exocytosis probably by liberating Ca2+ from intracellular stores. |
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