A gradient of poly(A)+ RNA sequences in Xenopus laevis eggs and embryos |
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Authors: | Clifford D. Carpenter William H. Klein |
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Affiliation: | Program in Molecular, Cellular, and Developmental Biology, Department of Biology, Indiana University, Bloomington, Indiana 47405 USA |
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Abstract: | Xenopus laevis eggs and gastrula stage embryos were fractionated into three equal sections normal to the animal-vegetal axis, and poly(A)+ RNA was isolated from each section. Hybridization of these poly(A)+ RNAs with [32P]cDNA synthesized using animal or vegetal poly(A)+ RNAs showed no detectable differences in the extents or rates of reaction. Thus, the vast majority of poly(A)+ RNAs are not segregated along the animal-vegetal axis. To increase the sensitivity of these experiments, [32P]cDNAs were prepared which had reduced levels of RNA sequences from the animal region of the gastrula stage embryo or spawned unfertilized egg. Hybridization reactions with these probes showed that 3 to 5% of the input cDNA represents poly(A)+ RNA sequences enriched 2- to 20-fold in the vegetal region of the egg or gastrula stage embryo. |
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