Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities |
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| Authors: | Montserrat Orencio-Trejo Noemí Flores Adelfo Escalante Georgina Hernández-Chávez Francisco Bolívar Guillermo Gosset Alfredo Martinez |
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| Affiliation: | Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor,, México. mon@ibt.unam.mx |
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| Abstract: | Background A metabolic regulation study was performed, based upon measurements of enzymatic activities, fermentation performance, and RT-PCR analysis of pathways related to central carbon metabolism, in an ethanologenic Escherichia coli strain (CCE14) derived from lineage C. In comparison with previous engineered strains, this E coli derivative has a higher ethanol production rate in mineral medium, as a result of the elevated heterologous expression of the chromosomally integrated genes encoding PDC Zm and ADH Zm (pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis). It is suggested that this behavior might be due to lineage differences between E. coli W and C. |
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