Direct evidence for the lack of methylation of two pulse labeled plant RNAs |
| |
Authors: | Posner, Herbert B. Gressel, Jonathan Rosner, Arie |
| |
Affiliation: | Department of Plant Genetics, The Weizmann Institute of Science Rehovot, Israel |
| |
Abstract: | A product of the processing of precursor rRNA ("excess" RNA)has been indirectly found to be unmethylated in mammalian systems,but direct measurement was precluded because of its instability.The "excess" RNA of duckweeds is relatively stable allowingdirect estimation of its methylation by polyacrylamide gel electrophoresis.The "excess" RNA with an apparent molecular weight 0.5?106 wasunmethylated. A pulse labeled RNA with an apparent molecularweight of 1.2?106 (presumably from chloroplasts) was also unmethylated.Under similar conditions the presumed cytoplasmic rRNA precursors,and the mature cytoplasmic and chloroplast rRNAs were methylated. 1Present address: Department of Biological Sciences, S.U.N.Y.Binghamton, N.Y. 13901, U.S.A. (Received May 9, 1974; ) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|