A new in vivo metabolite of vitamin D3: 1,25,26-trihydroxyvitamin D3 |
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Authors: | T A Reinhardt J L Napoli D C Beitz E T Littledike R L Horst |
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Affiliation: | Department of Biological Sciences University of Illinois at Chicago Circle Chicago, Illinois 60680 USA |
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Abstract: | The enzymatic conversion of 5-methylthioribose to methionine and its deaminated derivatives, 2-keto-4-methylmercaptobutyric acid and 2-hydroxy-4-methylmercaptobutyric acid by cell-free extracts of has been demonstrated. 14C-Labeled methionine was isolated from incubation mixtures with 5-methylthio[U-14C]ribose. The carbohydrate part of this compound furnishes at least part, if not all, of the four carbon chain of methionine. |
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Keywords: | HPLC high-pressure liquid chromatography |
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